By Professor Rudolf Rigler, Professor Elliot S. Elson (auth.)
This ebook provides the theoretical heritage to fluorescence correlation spectroscopy (FCS) and various purposes in quite a few fields of technology. FCS relies at the detection of unmarried molecules excited to fluorescence in diffraction restricted confocal quantity components and the time correlation of stochastic occasions. It presents final sensitivity within the research of molecular strategies and has chanced on a variety of functions in physics, chemistry and especially in biomolecular sciences. Its excessive spatial and temporal solution has made FCS a robust device for the research of molecular interactions and kinetics, shipping houses as a result of thermal movement and circulation, in addition to the physics of the excited nation in resolution in addition to on the mobile point. Its program in excessive throughput drug screening is utilizing all of the power of this major analytical tool.